Molecular mechanisms of the neuronal production and migration in the adult zebrafish brain

成年斑马鱼大脑神经元产生和迁移的分子机制

• 批准号: 20509005
• 负责人: KISHIMOTO Norihito
• 金额: $ 2.52万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20509005/
• 关键词: 再生医学; 神経科学; 脳・神経; 脳神経疾患; 発生・分化

To provide new insights into the cellular and molecular mechanisms of adult neurogenesis and neuronal migration, we have studied neuronal production and migration in the adult zebrafish telencephalon. (1) Using systematic immunohistochemical and ultrastructural studies, we defined the cellular composition of the ventricular zone (VZ) in the adult zebrafish telencephalon. (2) We provided direct evidence that neuronal precursors migrate from the telencephalic VZ to the olfactory bulb in the adult zebrafish brain using a transgenic zebrafish line that expresses GFP in neuronal precursors. (3) We established a novel zebrafish model to study injury-induced neurogenesis in the adult brain.

为了对成年神经发生和神经元迁移的细胞和分子机制提供新的见解，我们研究了成年斑马鱼端脑中神经元的产生和迁移。(1)利用系统的免疫组织化学和超微结构研究，我们确定了成年斑马鱼端脑脑室区（VZ）的细胞组成。(2)我们提供了直接的证据表明，神经元前体迁移从端脑VZ的嗅球在成年斑马鱼大脑中使用的转基因斑马鱼线，表达GFP的神经元前体。(3)我们建立了一种新的斑马鱼模型来研究成年脑损伤诱导的神经发生。

项目成果

Cystic kidney gene seahorse regulates cilia-mediated processes and Wnt pathway

囊性肾基因海马调节纤毛介导的过程和Wnt通路

• 发表时间: 2008
• 期刊: Developmental Cell Vol.14, No.6
• 作者: Kishimoto N.;Cao Y.;Park A.;Sun Z.
• 通讯作者: Sun Z.

The Cellular Composition of the Ventricular Zone and Neuronal Migration in the Adult Zebrafish Telencephalon

成年斑马鱼端脑心室区的细胞组成和神经元迁移

• 发表时间: 2010
• 作者: Kishimoto;N.;Alfaro-Cervello;C.;Shimizu;K.;Garcia-Verdugo;J.M.;Sawamoto;K.
• 通讯作者: K.

A Zebrafish Model to Study Adult Brain Injury and Regeneration

研究成人脑损伤和再生的斑马鱼模型

• 发表时间: 2010
• 作者: Kishimoto;N.;Shimizu;K.;Sawamoto;K.
• 通讯作者: K.

The Cellular Composition and Morphological Organization of the Subventricular Zone in the Adult Zebrafish Telencephalon

成年斑马鱼端脑室下区的细胞组成和形态组织

• 发表时间: 2009
• 作者: Kishimoto N;et al
• 通讯作者: et al

Injury-induced Activation of Cell Proliferation in the Adult Zebrafish Telencephalon

成年斑马鱼端脑损伤诱导的细胞增殖激活

• 发表时间: 2010
• 作者: Keisuke Ota;Takamasa Tsunoda;Toshiaki Omori;Shigeo Watanabe;Hiroyoshi Miyakawa;Masato Okada and Toru Aonishi;加藤茂弘;岸本憲人
• 通讯作者: 岸本憲人