Study on microbial community in tidal flat sediment sampled from Shirakawa- and Midorikawa-river.

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Redox-balanced circulation of nitrogen and sulfur will occur in a tidal flat where both aerobic and anaerobic conditions are shown by changing tide level. Bacteria should take important role in the elemental circulation and unbalanced circulation changes environmental condition worse. We analyzed microbial community in tidal flat sampled from Shirakawa- and Midorikawa-river. From the results in clone analysis targeted to 16S rRNA gene, both aerobic and anaerobic bacteria were detected in all samples and Alphaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Chloroflexi, Bacteroidetes and Actinobacteria were dominant. However, Thiobacillus group was dominant by the analysis targeted to functional genes such as aprA and nirS.