A new approach for sample preparation : expression and stable-isotope labeling of mature proteins using plant cells

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We developed a novel protein expression method using plant cells and virus vector. We prepared a protein, which cannot be expressed with popular E.coli systems. We established a protocol for preparing the stable-isotope labeled proteins with this system. To show the workability, we performed protein expression, stable-isotope labeling, and structure determination of a protein, of which expression is tough. In summary, this work had suggested a new method, which has a potential to study challenging proteins, to prepare samples, of which expression is known as difficult.