Synthesis of Cello-Oligosaccharides for Alcohol Fermentation by Hydrolysis of Crystalline-Region Cellulose

找到了一种实用的结晶纤维素水解法。水中的结晶纤维素悬浮液在260℃±10℃的温度下加热，氢键被削弱，然后进行蒸汽爆炸。制得的纤维素酶仅在其表面发生了水解。丝光纤维素也表现出同样的现象。对于转变为不同晶态的结晶纤维素，在200℃左右的酸性水解会得到单糖和低聚糖的衍生物，这表明主链发生了断裂。转化后的纤维素很容易被酶降解成单糖和低聚糖。

A practical method to hydrolyze crystalline cellulose was found out. After crystalline cellulose suspension in water was heated at 260℃±10℃ in which temperature the hydrogen bond is weakened, it was steam-exploded. The obtained cellulose showed that hydrolysis occurred only at its surface. Mercerized cellulose showed the same phenomenon. For the crystalline cellulose converted into a different crystalline state, an acidic hydrolysis around 200℃ gave mono- and oligo-saccharide derivatives, indicating the occurrence of main-chain scission. The converted cellulose was easily hydrolyzed by enzymes into monoand oligo-saccharides.