Creation of Excellent Mutant Enzymes and Synthetic Application

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The catalytic function of versatile biocatalysts such as lipase and carbonyl reductase were improved by rational design or random mutagenesis, and useful optically active compounds were synthesized. (1) Catalytic activity and enantioselectivity of lipase toward poor substrates have been dramatically improved by rational design. (2) The bioreduction ofα,α-difluorinated β-keto ester with recombinant E. coli gave the corresponding optically active alcohol, which was converted into α,α-difluorinated β-amino acid derivative.