IR spectroscopic analysis on sugar metabolic kinetics of suspension plant cells for efficient utilization of biomass materials

悬浮植物细胞糖代谢动力学的红外光谱分析，促进生物质材料的高效利用

• 批准号: 20560729
• 负责人: SUEHARA Ken-ichiro
• 金额: $ 2.41万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20560729/
• 关键词: 懸濁植物細胞; 赤外・近赤外分光法; 再資源化糖; バイオマス; 植物細胞培養; 糖代謝; 赤外分光法; 速度論的解析; バイオマス由来の糖

The objective of this work was to study the influence of sugar spices in the culture media on the kinetic sugar uptake phenomena for three types of plant cell cultivation. We developed the measurement method of sugar content in culture media suspended plant cells using the FT-IR/ATR method, and the sugar uptake rate of the suspension plant cells were calculated. The sugar uptake rate of the plant cell was different greatly on the kind of sugar, however, characteristic of sugar metabolism based on cell growth period was stable. Characteristic of metabolism of various sugars and sugar mixture (that is biomass degradation product) for three types of plant cells were obtained. These results will contribute to the development of a plant cell cultivation process planning and bioprocess monitoring by IR method to utilize the sugar derived from biomass materials.

本工作的目的是研究培养基中的糖香料对三种类型的植物细胞培养的糖吸收动力学现象的影响。建立了用FT-IR/ATR法测定植物悬浮细胞培养基中糖含量的方法，并计算了植物悬浮细胞的糖吸收速率。植物细胞对糖的吸收速率因糖的种类而有较大差异，但基于细胞生长期的糖代谢特征是稳定的。获得了三种植物细胞对各种糖和糖混合物（即生物质降解产物）的代谢特性。这些结果将有助于开发一个植物细胞培养过程规划和生物过程监测的红外方法，利用糖来源于生物质材料。

项目成果

Infrared spectral feature of plant-cell culture media

植物细胞培养基的红外光谱特征

• 发表时间: 2010
• 期刊: Proceedings of 14th international conference of near infrared spectroscopy
• 作者: K Suehara;A Hashimoto
• 通讯作者: A Hashimoto

中赤外分光法を援用した懸濁植物細胞の糖代謝挙動に及ぼすグルコース前培養の影響評価

利用中红外光谱评估葡萄糖预孵育对悬浮植物细胞糖代谢行为的影响

• 发表时间: 2011
• 作者: 橋詰直幸;櫻井明彦;榊原三樹男;末原憲一郎・川口摩悠美・亀岡孝治・橋本篤
• 通讯作者: 末原憲一郎・川口摩悠美・亀岡孝治・橋本篤

中赤外分光法を援用した懸濁アラビドプシス細胞の糖代謝挙動の速度論的検討

利用中红外光谱研究悬浮拟南芥细胞糖代谢行为的动力学

• 发表时间: 2010
• 作者: 末原憲一郎・江川芳美・酒井麻衣・亀岡孝治・橋本篤

Grasping of Spectral Feature on MIR and NIR Regions for Analysis of Plant-cell Culture Broth

掌握中红外和近红外区域的光谱特征以分析植物细胞培养液

• 发表时间: 2009
• 作者: 末原憲一郎;橋本篤
• 通讯作者: 橋本篤