IR spectroscopic analysis on sugar metabolic kinetics of suspension plant cells for efficient utilization of biomass materials

本文研究了三种植物细胞培养培养基中添加糖香料对细胞动态摄糖现象的影响。建立了用FT-IR/ATR法测定培养基悬浮植物细胞中糖含量的方法，并计算了悬浮植物细胞的糖吸收率。不同种类的糖，植物细胞的糖吸收速率差异较大，但基于细胞生长期的糖代谢特征是稳定的。获得了三种类型植物细胞对各种糖和糖混合物（即生物量降解产物）的代谢特性。这些结果将为利用生物质原料中提取的糖进行植物细胞培养过程规划和生物过程监测提供参考。

The objective of this work was to study the influence of sugar spices in the culture media on the kinetic sugar uptake phenomena for three types of plant cell cultivation. We developed the measurement method of sugar content in culture media suspended plant cells using the FT-IR/ATR method, and the sugar uptake rate of the suspension plant cells were calculated. The sugar uptake rate of the plant cell was different greatly on the kind of sugar, however, characteristic of sugar metabolism based on cell growth period was stable. Characteristic of metabolism of various sugars and sugar mixture (that is biomass degradation product) for three types of plant cells were obtained. These results will contribute to the development of a plant cell cultivation process planning and bioprocess monitoring by IR method to utilize the sugar derived from biomass materials.