Molecular mechanism of genome maintenance in Thermus thermophilus

制备了参与错配修复和碱基切除修复系统的嗜热热菌HB8蛋白，并进行了修复反应重构、蛋白-蛋白相互作用分析和晶体结构分析。基于这些结果，我揭示了错配修复蛋白之间的新相互作用有助于链辨别，降解错误链的核酸酶通过其独特的结构识别单链DNA，以及两种DNA聚合酶在碱基切除修复的不同途径中起作用。

I prepared Thermus thermophilus HB8 proteins involved in mismatch repair and base excision repair systems and performed reconstitution of repair reactions, protein-protein interaction analysis and crystal structural analyss. Based on the results, I revealed that novel interactions between mismatch repair proteins contribute to strand discrimination, the nuclease which degrades an error-strand recognizes single-straded DNA by its unique structure, and two DNA polymerases function in different pathways of base excision repair.