Functional analysis of microRNAs on sex determination and differentiation

为了研究microRNA是否在性腺形成和性分化中起作用，采用高灵敏的microRNA图谱分析方法MRAP，以从雄性和雌性性腺中纯化的小鼠胚胎支持细胞为材料，测定了microRNA图谱。用TaqMan方法对得到的图谱进行了进一步验证。结果表明，mir-199a-5p在雌性支持细胞中优先表达，提示microRNA与小鼠胚胎性腺支持细胞的性腺形成和性分化有关。

To examine if microRNA plays a role in gonad formation and sexual differentiation, microRNA profiles were determined by high-sensitive microRNA profiling method, mRAP, using mouse embryonic supporting cells purified from male and female gonads. The profiles obtained were further verified by TaqMan method. The results shows that mir-199a-5p is preferentially expressed in female supporting cells, suggesting that microRNA is associated with gonad formation and sexual differentiation in supporting cells in mouse embryonic gonad.