Improvement of the saprophytic ability in Matsutake mushroom by cross breeding technology

鉴定了几个与交配型和淀粉代谢相关的基因，用于研究松茸菌株的交配型和核型。这些结果表明，从松茸子实体组织分离的松茸基本上是异核体。其次，对外生菌根真菌松茸的原生质体形成和再生条件进行了研究，以期分离出同核体。结果表明，海藻糖作为渗透稳定剂，促进了松茸原生质体的形成和菌丝的生长，而木糖则完全抑制了菌丝的生长。

Several genes related for mating type and starch metabolism were identified to use for investigation of mating type and karyotype in the T.matsutake isolates. From these results indicated that the T.matsutake isolates from the fruiting body tissue were essentially heterokaryon. Secondly, we examined the condition of protoplast formation and its regeneration in order to isolate homokaryon in the ectomycorrhizal fungus Tricholoma matsutake.As the results, when trehalose was used as osmotic stabilizer, the protoplast formation and mycelial growth of T.matsutake was promoted, however, xylose completely inhibited mycelial growth.