Development of the safe and functional avirulent neurotracing virus producing system.

测定了猪嗜神经冠状病毒(PHEV)67N株的全基因组序列，为PHEV反向遗传学发展基于PHEV的神经示踪病毒奠定了基础。嗜神经性67N株NS2基因约有400个碱基插入，非嗜神经性OSN204株NS2基因约有200个碱基缺失。PHEV对神经母细胞瘤和神经胶质瘤细胞株的体外感染结果表明，PHEV 67N株具有高度的神经趋化性。

The entire genome sequence of the porcine hamagglutinating encephalomyelitis virus (PHEV) 67N strain, neurotropic coronavirus, was determined, and the sequence data is useful for reverse genetics of PHEV to develop the PHEV based neurotracing virus. There is about 400 base insertion in ns2 gene of neurotropic 67N strain and 200 base deletion in ns2 gene of non neurotropic OSN204 strain. The results of in vitro PHEV infection to Neuroblastoma and glioma cell line indicated that PHEV 67N strain have high neurotropism.