Development of the safe and functional avirulent neurotracing virus producing system.

开发安全且功能性的无毒神经追踪病毒生产系统。

• 批准号: 20580319
• 负责人: TANIGUCHI Takahide
• 金额: $ 3.08万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580319/
• 关键词: ウイルス; 脳・神経; バイオテクノロジー; ニューロトレーサー

The entire genome sequence of the porcine hamagglutinating encephalomyelitis virus (PHEV) 67N strain, neurotropic coronavirus, was determined, and the sequence data is useful for reverse genetics of PHEV to develop the PHEV based neurotracing virus. There is about 400 base insertion in ns2 gene of neurotropic 67N strain and 200 base deletion in ns2 gene of non neurotropic OSN204 strain. The results of in vitro PHEV infection to Neuroblastoma and glioma cell line indicated that PHEV 67N strain have high neurotropism.

测定了猪嗜神经冠状病毒(PHEV)67N株的全基因组序列，为PHEV反向遗传学发展基于PHEV的神经示踪病毒奠定了基础。嗜神经性67N株NS2基因约有400个碱基插入，非嗜神经性OSN204株NS2基因约有200个碱基缺失。PHEV对神经母细胞瘤和神经胶质瘤细胞株的体外感染结果表明，PHEV 67N株具有高度的神经趋化性。

项目成果

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