Analysis of collagen fibril formation and degeneration by atomic force microscopy in liquid condition

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In the present study, we investigated that the mechanism of formation and degeneration of collagen fibrils by atomic force microscopy(AFM) in liquid condition. The results of the study are as follows.1) We succeeded in the observation of segment-long-spacing(SLS) crystallites, which is the bundle of type I collagen molecules, by atomic force microscopy in liquid.2) A human osteosarcoma cell line named NOS-1 is known to produce a large amount of type I collagen fibrils on the culture dish. The developing collagen fibrils were observed by AFM in phosphate-buffered saline.3) We succeeded in the observation of collagen fibrils produced by NOS-1with high-speed AFM.