Identification of genes involved in the vascular network formation though neuro-vascular interactions

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We identified genes which were reproducibly up-regulated in HMVEC by the co-culture with dorsal root ganglion neurons and human microvascular endothelial cells (HMVEC). We found that hypoxia down-regulated soluble VEGF receptor-1 (soluble Flt-1, sFlt-1) expression in HMVEC and identified cis-elements that regulated sFlt-1 mRNA 3'-end processing. We found significantly reduced expression of esRAGE, a decoy receptor for amyloid β, in the hippocampal tissues from Alzheimer's disease brains by immunohistochemical analysis. We revealed that HMVEC expressed over 20 microRNAs including miR21. We injected lentiviral vectors into embryonic mouse skin and brain, and confirmed the expression of the introduced genes. We developed an assay method for esRAGE mRNA and an esRAGE transgenic mouse, and applied for patents for them.