3D nuclear architecture of the regions close to the candidate gene (s) related to the constitutional chromosomal abnormalities

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3D-FISH analyses performed to measure the relative positioning of SNRPN and UBE3A genes at 15q11.2 region on each homologous chromosomes 15 within the interphase nucleus of normal B-lymphoblastoid cell lines. The average distances between two genes on each chromosome 15 were 0.32 and 0.60μm. This result suggests the 3D distance between two genes on one of the chromosome 15 seems to be different from on the other chromosome 15 in each nucleus. The minimal distances from the SNRPN genes to the nuclear membrane were 0.6±0.7 and 1.4±0.9μm, and also seems to be different between the homologous chromosomes.