Analysis of lipid and apolipoprotein reflecting clinical condition of inflammatory bowel disease by mass spectrometry

为了研究一种反映炎症性肠病临床状况的生化标志物的快速测量系统及其在实验室研究中的应用，我们分析了人血清中脂质和蛋白质代谢分子异常的浓度、组成和结构。我们建立了用基质相关电离飞行时间质谱（MALDI-TOF-MS）、气相色谱-电子冲击质谱（GC/MS）和高效液相色谱（HPLC）分析磷脂及其代谢物，以及含硫氨基酸、半胱氨酸和同型半胱氨酸修饰蛋白的方法。患者炎症指数高，但脂质、载脂蛋白、含硫氨基酸水平低，磷脂呈特征性组成。

For investigation of a rapid measurement system and its application to a laboratory study of the biochemical markers reflecting the clinical condition of inflammatory bowel disease, we analyzed the concentration, composition, and structure of molecular abnormalities of lipid and protein metabolism in human serum. We established methods for analysis of phospholipids and their metabolites, as well as modified protein with sulfur-containing amino acids, cysteine, and homocysteine, using matrix-associated ionization time-of-flight mass spectrometry (MALDI-TOF-MS), gas chromatography electron impact mass spectrometry (GC/MS), and high-performance liquid chromatography (HPLC). The inflammation index of the patient was high, but levels of lipids, apoproteins, and sulfur-containing amino acids were low, and the phospholipids showed a characteristic composition.