Improved function of ketoamine oxidase for clinical examination reagent of diabetes by use of multiple approaches

多途径提高糖尿病临床检验试剂酮胺氧化酶的功能

• 批准号: 20590579
• 负责人: UEDA Tadashi
• 金额: $ 3.16万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590579/
• 关键词: 糖尿病; ケトアミンオキシダーゼ; アミノ酸改変; X線結晶解析; 安定化; 酵素; 機能向上; 酵素の高機能化; ジスルフィド結合; タンパク質の安定化; Gly-Pro配列; グリコアルブミン

For prevention of diabetes, we should control of the concentration of glucose in our vessels. Since the concentration of glucose is affected by foods, the concentration of glycated proteins in vessels often became used as an indicator of the average of concentration of glucose. The ketoamine oxidase is crucial for the evaluation of the concentration of glucose from the glycated proteins. To improve the function of the ketoamine oxidase, we prepared many ketoamine oxidase mutants but did not obtain ketoamine oxidase mutant with improved function. Then, in order to obtain the basis for mutation design, we tried to analyze the three-dimensional structure of the ketoamine oxidase in vain.

为了预防糖尿病，我们应该控制血管中的葡萄糖浓度。由于葡萄糖的浓度受食物的影响，血管中糖化蛋白的浓度经常被用作葡萄糖平均浓度的指标。酮胺氧化酶对于评估糖化蛋白质中葡萄糖的浓度是至关重要的。为了提高酮胺氧化酶的功能，我们制备了许多酮胺氧化酶突变体，但没有获得功能增强的突变体。然后，为了获得突变设计的依据，我们尝试分析了酮胺氧化酶的三维结构，但没有成功。

项目成果

Gly-Pro 配列導入によるケトアミンオキシダーゼの安定性評価

引入Gly-Pro序列评价酮胺氧化酶的稳定性

• 发表时间: 2010
• 作者: 末吉彩;植田正;他
• 通讯作者: 他

A Protein's Conformational Stability Is an Immunologically Dominant Factor: Evidence That Free-Energy Barriers for Protein Unfolding Limit the Immunogenicity of Foreign Proteins

• DOI: 10.4049/jimmunol.0902249
• 发表时间: 2010-10-01
• 期刊: JOURNAL OF IMMUNOLOGY
• 影响因子: 4.4
• 作者: Ohkuri, Takatoshi;Nagatomo, Satoko;Ueda, Tadashi
• 通讯作者: Ueda, Tadashi

Effect of His mutants on the fibrillation of amyloidogenic Vλ6 protein under acidic and physiological conditions

酸性和生理条件下His突变体对淀粉样变Vλ6蛋白纤维化的影响

• 发表时间: 2010
• 期刊: Biochem.Biophys.Res.Commun. 391
• 作者: Mishima;Ueda;et al.
• 通讯作者: et al.

Crystal structure of K33 mutant hen lysozymes with enhanced activities.

活性增强的 K33 突变母鸡溶菌酶的晶体结构。

• 发表时间: 2008
• 期刊: The Journal of Biochemistry 144
• 作者: Goto T.;Ohkuri T.;Shioi S.;Abe;Y.;Imoto T.;Ueda.
• 通讯作者: Ueda.

Residual Structures in Acid-Unfolded States of Vλ6 Proteins Affect Amyloid Fibrillation

Vλ6 蛋白酸解折叠状态的残余结构影响淀粉样纤维颤动

• 发表时间: 2009
• 期刊: Journal of Molecular Biology 392
• 作者: Mishima;Ueda;et al.
• 通讯作者: et al.