Development of diagnostic and therapeutic strategies for gastrointestinalcancer : focus on tumor-associated ribosomal proteins

胃肠癌诊断和治疗策略的发展：关注肿瘤相关核糖体蛋白

• 批准号: 20590746
• 负责人: SASAKI Yasushi
• 金额: $ 2.91万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590746/
• 关键词: リボゾーム蛋白質; 消化器癌; p53; 抗癌剤感受性; アポトーシス; リボソーム蛋白質

We report here that L13 is activated in human gastrointestinal cancers. By using qRT-PCR, we analyzed expression of L13 in freshly resected cancer tissue of the stomach, colorectum, and liver. Upregulation of L13 mRNA expression was observed in 10 (28%) of 36 stomach, 19 (41%) of 46 colorectal, and 5 (20%) of 25 liver cancer tissue samples compared to adjacent normal tissue samples. We also found that increased expression of the L13 gene was correlated with clinical staging in stomach cancers. Importantly, we discovered that L13 expression was inversely related to p53 mutational status in primary gastric cancer, and siRNA-mediated L13 suppression resulted in robust induction of p53 protein. These findings suggest that L13 plays an essential role in the progression of some gastrointestinal malignancies by numbing the p53 pathway, thus allowing tumorigenic growth in the presence of wild-type p53.

我们在此报告L13在人类胃肠道癌症中被激活。通过qRT-PCR，我们分析了L13在新切除的胃、结直肠和肝脏癌组织中的表达。与癌旁正常组织相比，36例胃癌组织中有10例(28%)、46例结直肠癌组织中有19例(41%)、25例肝癌组织中有5例(20%)表达上调。我们还发现，L13基因的表达增加与胃癌的临床分期有关。重要的是，我们发现L13在原发胃癌中的表达与P53突变状态呈负相关，siRNA介导的L13抑制导致P53蛋白的强烈诱导。这些发现表明，L13通过麻木P53途径，从而在野生型P53存在的情况下允许肿瘤生长，从而在一些胃肠道恶性肿瘤的进展中发挥重要作用。

项目成果

Myogenic lineage differentiated mesenchymal stem cells enhance recovery from dextran sulfate sodium-induced colitis in the rat

• DOI: 10.1007/s00535-010-0320-7
• 发表时间: 2011-02
• 期刊: Journal of Gastroenterology
• 影响因子: 6.3
• 作者: Hiroki Tanaka;Y. Arimura;Takashi Yabana;A. Goto;M. Hosokawa;Kanna Nagaishi;K. Yamashita;Hiroyuki Yamamoto;Y. Sasaki;M. Fujimiya;K. Imai;Y. Shinomura

Genomic Screening for Genes Silenced by DNA Methylation Revealed an Association between RASD1 Inactivation and Dexamethasone Resistance in Multiple Myeloma

• DOI: 10.1158/1078-0432.ccr-08-3336
• 发表时间: 2009-07-01
• 期刊: CLINICAL CANCER RESEARCH
• 影响因子: 11.5
• 作者: Nojima, Masanori;Maruyama, Reo;Shinomura, Yasuhisa
• 通讯作者: Shinomura, Yasuhisa

A Single Recombinant Adenovirus Expressing p53 and p21-targeting Artificial microRNAs Efficiently Induces Apoptosis in Human Cancer Cells

• DOI: 10.1158/1078-0432.ccr-08-2396
• 发表时间: 2009-06-01
• 期刊: CLINICAL CANCER RESEARCH
• 影响因子: 11.5
• 作者: Idogawa, Masashi;Sasaki, Yasushi;Tokino, Takashi
• 通讯作者: Tokino, Takashi

hCLCA2, a p53 inducible transmembrane protein regulates cancer cell migration and adhesion.

hCLCA2 是一种 p53 诱导型跨膜蛋白，可调节癌细胞迁移和粘附。

• 发表时间: 2010
• 作者: 佐々木泰史;横田育子;鹿島理沙;井戸川雅史;鈴木拓;豊田実;今井浩三;篠村恭久;時野隆至
• 通讯作者: 時野隆至

N-propionyl-cysteaminylphenol-magnetite conjugate(NPrCAP/M)is a novel nanoparticle for the targeted growth suppression of melanoma cells

N-丙酰基半胱氨基苯酚-磁铁矿缀合物（NPrCAP/M）是一种新型纳米粒子，用于靶向抑制黑色素瘤细胞的生长

• 发表时间: 2009
• 期刊: J Invest Dermatol 129
• 作者: Sato M;et al
• 通讯作者: et al