Search and validation of diagnostic marker for nonalcoholic steatohepatitis using proteomics.

首先，我们利用蛋白质组学方法对氧化应激标志物(S)进行了研究，发现其可能是鉴别非酒精性脂肪性肝病患者中单纯性脂肪炎和非酒精性脂肪性肝炎的有用的血清生物标志物。第二，在一个大鼠实验模型中，高果糖饮食(H-FRUC)组发生了肝脏脂肪变性(HS)，但与高脂肪饮食(H-FAT)组相比没有那么严重。相反，与H-FAT组和对照组相比，H-FRUC组70%部分肝切除(PHX)后的肝再生受损。此外，与H-FAT组相比，H-FRUC组发生GST-P阳性癌前病变的发生率显著更高。这些结果表明，PHX后肝再生障碍和HS癌前病变的发生率与脂肪变性的原因有关，而不是与程度有关。因此，我们的研究表明，血清MnSOD是NAFLD潜在的新的标志物，不同的饮食可能会影响NAFLD的病理生理状况和进展。

First of all, we explored the oxidative stress marker (s) using proteomics and identified that MnSOD may be a useful serum biomarker for distinguishing between simple steatotis (SS) and nonalcoholic steatohepatitis (NASH) in patients with nonalcoholic fatty liver disease (NAFLD). Second, in a rat experimental model, hepatic steatosis (HS) developed in the high fructose diet (H-fruc) group, but was less severe compared to the high fat diet (H-fat) group. In contrast, liver regeneration after 70% partial hepatectomy (PHx) was impaired in the H-fruc group compared to the H-fat and control groups. In addition, the H-fruc group developed a significantly higher incidence of GST-P-positive precancerous lesions compared to the H-fat group. These results indicate that impairment of liver regeneration after PHx and the incidence of precancerous lesions in HS are related to the cause, rather than the degree, of steatosis. Thus, our study demonstrates that serum MnSOD is potential novel marker for NAFLD, and that the pathophysiological condition and progression of NAFLD are potentially affected by different diets.