Organellar dynamics in plant male gametophyte visualized by fluorescent live imaging

将线粒体靶向荧光蛋白与营养细胞或精子细胞特异性表达相结合，使我们能够观察到线粒体在各自细胞类型中的精确数量和动态行为。此外，实时成像分析显示精子线粒体进入卵子和中心细胞。我们发现，本项目构建的转基因材料不仅可以用于实时成像，而且可以通过突变体分析来研究线粒体功能。

Combination of mitochondria-targeted fluorescent proteins with vegetative or sperm cell-specific expression allowed us to observe precise number and dynamic behavior of mitochondria in the respective cell types. Furthermore, live imaging analysis showed that sperm mitochondria enter into egg and central cells. We showed that the transgenic materials constructed in this project are useful for not only live imaging but also studying mitochondrial functions by mutant analysis.