Study of regulatory mechanism of intracellular vesicle transport by motor proteins using 3-dimensional tracking of nano-particle.

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Motor proteins play an important role in transporting organelles and vesicles to the destination. The goal of this project is to construct new system to understand the regulatory mechanism of motor-based intracellular transport. I purified recombinant motor proteins, and could obtain fluorescent nano-particles attached to these proteins. The nano-particles were induced to semi-intact cells, and I could successfully track the 3-dimensional movement of these in high speciotemporal resolution.