Study of regulatory mechanism of intracellular vesicle transport by motor proteins using 3-dimensional tracking of nano-particle.

利用纳米颗粒的 3 维跟踪研究运动蛋白对细胞内囊泡运输的调节机制。

• 批准号: 20770116
• 负责人: TAKETOSHI Kambara
• 金额: $ 2.66万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770116/
• 关键词: モータータンパク質; 細胞内輸送

Motor proteins play an important role in transporting organelles and vesicles to the destination. The goal of this project is to construct new system to understand the regulatory mechanism of motor-based intracellular transport. I purified recombinant motor proteins, and could obtain fluorescent nano-particles attached to these proteins. The nano-particles were induced to semi-intact cells, and I could successfully track the 3-dimensional movement of these in high speciotemporal resolution.

运动蛋白在将细胞器和囊泡运输到目的地方面发挥着重要作用。该项目的目标是构建新的系统来了解基于运动的细胞内运输的调节机制。我纯化了重组运动蛋白，并可以获得附着在这些蛋白上的荧光纳米颗粒。纳米颗粒被诱导形成半完整的细胞，我可以成功地以高光时分辨率追踪这些细胞的 3 维运动。