Single-molecule imaging of DNA―protein interactions in DNA repair

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To elucidate fundamental processes of DNA-protein interactions in DNA repair, we have performed single-molecule imaging of DNA-protein and protein-protein interactions using fluorescently labeled proteins involved in nucleotide excision repair and mismatch repair in Escherichia coli. The single-molecule observation in this study reveals DNA binding modes of a helicase and the number of the helicase bound to DNA.