Identification of regulators controlling cellulase and hemicellulase gene expression in filamentous fungi and its functional analysis.
Identification of regulators controlling cellulase and hemicellulase gene expression in filamentous fungi and its functional analysis.
批准号:
20780058
负责人:
TANI Shuji
金额:
$2.75万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2009
中文摘要
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英文摘要
We report the successful Agrobacterium tumefaciens-mediated transformation (AMT) of Aspergillus aculeatus for functional genomics. We isolated 40 transformants expected to be disruptants of the regulator(s) controlling cellulase gene expressions in A. aculeatus from 15,000 transformants obtained by AMT. Mutation of the geneA resulted in 5-fold reduction on cellobiohydrolase gene expression in transformant by comparing with that in host strain. Now, we are further analyzing the function of putative transcription factor identified in this study and seeking the other regulators.
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Aspergillus aculeatusにおける網羅的遺伝子破壊株の作出を目的としたアグロバクテリウム形質転換法の開発
开发用于生产棘孢曲霉综合基因破坏菌株的农杆菌转化方法
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[國武絵美, 谷修治, 炭谷順一, 川口剛司]
通讯作者:
川口剛司
Development of Agrobacterium tumefaciens-mediated transformation system for random insertional mutaaenesis in Aspergillus aculeatus
开发根癌农杆菌介导的棘孢曲霉随机插入诱变转化系统
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[E. Kunitake, S. Tani, J. Sumitani, and T. Kawaguchi]
通讯作者:
and T. Kawaguchi
Physiological inducer and cis-elements required for the cellobiohydrolase Igene induction via XlnR-independent pathway in Aspergillus aculeatus.
棘孢曲霉中通过 XlnR 独立途径诱导纤维二糖水解酶 I 基因所需的生理诱导剂和顺式元件。
DOI:
--
发表时间:
期刊:
Biotechnology of lignocelluloses degradation, biomassutilization, and biorefinery. 2009-11
影响因子:
--
作者:
[S.Tani, J.Sumitani, T.Kawaguchi]
通讯作者:
T.Kawaguchi
Physiological inducer and cis-elements required for the cellobiohydrolase lgene induction via XlnR-indenendent pathway in Aspergillus aculeatus
棘孢曲霉中通过 XlnR 独立途径诱导纤维二糖水解酶基因所需的生理诱导剂和顺式元件
DOI:
--
发表时间:
期刊:
Biotechnology of lignocelluloses degradation, biomass utilization, and biorefinery (In press)
影响因子:
--
作者:
[S. Tani, J. Sumitani, and T. Kawaguchi]
通讯作者:
and T. Kawaguchi
Development of Agrobacterium tumefaciens-mediated transformation system for random insertional mutagenesis in Aspergillus aculeatus
开发根癌农杆菌介导的棘孢曲霉随机插入诱变转化系统
DOI:
--
发表时间:
2009
期刊:
Biothechnology of Lignocellulose Degradation, Biomass Utilization, and Biorefinery
影响因子:
--
作者:
[Emi Kunitake, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi]
通讯作者:
Takashi Kawaguchi
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