The regulatory system of methanol metabolism by oxygen in methylotrophic yeast Pichia methanolica.

甲醇代谢途径是一个很好的氧反应系统模型，因为它需要和消耗氧气。甲醇营养型酵母Pichia methanolica的甲醇代谢过程中，呼吸链中大量的氧被用于甲醇的氧化和ATP的合成。本文研究了甲醇毕赤酵母CYC1的表达模式。PmCYC1的表达水平随着甲醇浓度的变化而变化，并且当在甲醇上生长时，CYC1的表达在高氧浓度下被强烈诱导。这些结果表明，PmCYC1的这种表达模式的变化是特异性的甲醇代谢活性。在真核生物中，染色质重塑系统在转录调控中起着重要作用。为了发现组蛋白乙酰化是否在AOD的调节中起作用，我们测试了组蛋白降解酶（HDAC）激活剂的作用。因此，HDAC的有效激活剂白藜芦醇诱导了AOD的表达。

Methanol metabolic pathway is a good model for oxygen response system as it requires and consumes oxygen. Methanol metabolism in methylotrophc yeast, Pichia methanolica a large amount of oxygen are used for methanol oxidation and for synthesis of ATP in respiratory chain. In this study the author investigated that Pichia methanolica CYC1 expression patterns. The level of PmCYC1 expression changed with methanol concentration, and CYC1 expression was induced strongly at high oxygen concentration when grown on methanol. These results showed that, this expression pattern change of PmCYC1 is specific to methanol metabolizing activity. In eukaryotes chromatin remodeling system is important in transcriptional regulation. To find out whether histone acetylation plays a role in the regulation of AOD we tested the effect of histone decelases (HDACs) activator. As a result the expression of AOD induced by Resveratrol which is a potent activator of HDAC.