Construction of D-aminoacyl peptides synthetase

引入D-氨基酸的肽有望增加肽的功能多样性和应用。在这项研究中，我们构建了突变的D-氨基肽酶作为一个方便的工具，合成肽纳入D-氨基酸。我们获得了一种丝氨酸D-立体专一性酰胺水解酶，它通过催化氨解反应而具有肽键形成活性。该酶利用D-脯氨酸衍生物作为酰基供体底物，以一锅法高效合成具有几丁质酶抑制活性的cyclo（D-脯氨酸-L-精氨酸）。此外，我们确定了残基，Asn 193，它与酰基供体侧链的识别，从突变分析。

Peptides incorporating D-amino acids are expected to increase the functional variety and applications of peptides. In this study, we constructed the mutant D-aminopeptidases as a convenient tool for synthesis of peptides incorporating D-amino acids. We obtained a serine D-stereospecific amidohydrolase that exhibit peptide bond formation activity by catalysis of aminolysis reaction. The enzyme utilizes D-Pro derivatives as acyl donor substrate, and exhibit efficient synthesis of cyclo (D-Pro-L-Arg) that has chitinase inhibitory activity in a one-pot reaction manner. In addition, we identified the residue, Asn193, which associates the recognition of side chain of acyl donor, from the mutation analysis.