Identification of fresh and seawater specific molecular markers in teleosts

硬骨鱼淡水和海水特异性分子标记的鉴定

• 批准号: 20780147
• 负责人: HIROI Junya
• 金额: $ 2.75万
• 依托单位: St.Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20780147/
• 关键词: 魚類; 環境適応; 浸透圧調節; 淡水; 海水; 塩類細胞; イオントランスポーター; イオン輸送体

Euryhaline fish can live in both fresh water and seawater by means of specialized "mitochondrion-rich cells (MRCs)". Although MRCs are considered to be involved in both ion uptake in fresh water and ion secretion in seawater, the ion-uptake mechanism has been controversial for over 30 years. In this study, we identified a novel freshwater-type ion transporter in tilapia MRCs and proposed a new ion uptake model. We also found that ion uptake mechanisms vary among fish species. These findings are opening a new window for the study on fish osmo- and iono-regulation.

广盐鱼可以通过特殊的“富线粒体细胞”（MRCs）在淡水和海水中生存。尽管MRCs被认为既参与淡水中的离子吸收，也参与海水中的离子分泌，但其离子吸收机制30多年来一直存在争议。本研究在罗非鱼MRCs中发现了一种新的淡水型离子转运体，并提出了一种新的离子摄取模型。我们还发现，不同鱼类的离子摄取机制各不相同。这些发现为研究鱼类的渗透和离子调节打开了一扇新的窗口。

项目成果

Different Hatching Strategies in Embryos of Two Species, Pacific Herring Clupea pallasii and Japanese Anchovy Engraulis japonicus, That Belong to the Same Order Clupeiformes, and Their Environmental Adaptation

• DOI: 10.1002/jez.b.21247
• 发表时间: 2009-03-15
• 期刊: JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
• 影响因子: 2.2
• 作者: Kawaguchi, Mari;Fujita, Hideaki;Yasumasu, Shigeki
• 通讯作者: Yasumasu, Shigeki

Morphological and functional classification of ion-absorbing mitochondria-rich cells in the gills of Mozambique tilapia

• DOI: 10.1242/jeb.025957
• 发表时间: 2009-04-01
• 期刊: JOURNAL OF EXPERIMENTAL BIOLOGY
• 影响因子: 2.8
• 作者: Inokuchi, Mayu;Hiroi, Junya;Kaneko, Toyoji
• 通讯作者: Kaneko, Toyoji

Osmo-and Ionoregulation. In Fish Larval Physiology (ed. R. N. Finn and B. G. Kapoor)

渗透和离子调节。

• 发表时间: 2008
• 作者: Kaneko;T. and Hiroi;J.(分担執筆)
• 通讯作者: J.(分担執筆)

Gene expression and morphological localization of NHE3, NCC and NKCC1a in branchial mitochondria-rich cells of Mozambique tilapia (Oreochromis mossambicus) acclimated to a wide range of salinity

适应各种盐度的莫桑比克罗非鱼（Oreochromis mossambicus）富含鳃线粒体的细胞中 NHE3、NCC 和 NKCC1a 的基因表达和形态定位

• 发表时间: 2008
• 期刊: Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology 151
• 作者: Inokuchi M;Hiroi J;Watanabe S;Lee KM;Kaneko T
• 通讯作者: Kaneko T

魚の浸透圧調節の謎に挑む. 稚魚学-多様な生理生態を探る(田申克編)

挑战鱼类渗透压调节的奥秘——探索鱼类的多样化生理生态（Katsu Tashin 编辑）

• 发表时间: 2008
• 作者: Kaneko T;Hiroi J;廣井準也(分担執筆)
• 通讯作者: 廣井準也(分担執筆)