Analysis of L(3)MBT transgenic mice

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It has been shown that epigenetic regulation of gene expression plays important roles in a variety of biological actions, such as cell proliferation, differentiation as well as maintenance of the purlipotency of the stem cells. In this study, we focused on L(3)MBT, one of the polycomb group proteins, and tried to investigate L(3)MBT deficient mice to know the physiological function of L(3)MBT and influences caused by its dysfunction. As conventional L(3)MBT-null mice died in the early embryonic phase, a conditional targeting vector which can be used for inducible deletion of the gene were prepared. ES cells which performed homologous recombination with the vector were obtained, and the chimeric mice were generated from the ES cells.