Elucidation of function of increasing toxin in the streptococcal toxic shock syndrome clinical isolates and of the regulation

链球菌中毒性休克综合征临床分离株增毒素功能及其调节的阐明

• 批准号: 20790345
• 负责人: IKEBE Tadayoshi
• 金额: $ 2.75万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790345/
• 关键词: 病原性; 劇症型溶連菌感染症; Streptococcus pyogenes

Streptococcal toxic shock syndrome(STSS) is a severe invasive infection characterized by the sudden onset of shock and multiorgan failure ; it has a high mortality rate. In the present study, mutations of another negative regulator, rgg, were also found in clinical isolates of STSS patients. The rgg mutants from STSS clinical isolates enhanced lethality and impaired various organs in the mouse models and precluded their being killed by human neutrophils. When we assessed the mutation frequency of rgg gene among S. pyogenes isolates from STSS and non-invasive infections, about 25% of the STSS isolates had mutations of rgg gene, while isolates from patients with non-invasive disease had significantly fewer mutations in this gene(1.7%). The results of the present study suggest that mutations in the negative regulators csrS/csrR and rgg of S. pyogenes are crucial factors in the pathogenesis of STSS, as they lead to the overproduction of multiple virulence factors.

链球菌中毒性休克综合征（STSS）是一种以突发性休克和多器官功能衰竭为特征的严重侵袭性感染；它的死亡率很高。在本研究中，在临床分离的STSS患者中也发现了另一种负调节因子rgg的突变。来自STSS临床分离株的rgg突变体在小鼠模型中增强了致死率，损害了各种器官，并阻止了它们被人类中性粒细胞杀死。当我们评估来自STSS和非侵袭性感染的化脓性链球菌分离株中rgg基因的突变频率时，大约25%的STSS分离株具有rgg基因突变，而来自非侵袭性疾病患者的分离株具有明显较少的该基因突变（1.7%）。本研究结果表明，化脓性链球菌负调控因子csrS/csrR和rgg的突变是STSS发病的关键因素，因为它们导致多种毒力因子的过量产生。

项目成果

Working Group for Beta-Haemolytic Streptococci in Japan. T serotyping of Streptococcus pyogenes in Aichi Prefecture, Japan isolated between 2003 and 2007

日本β-溶血性链球菌工作组。

• 发表时间: 2009
• 期刊: Jpn J Infect Dis
• 影响因子: 2.2
• 作者: Matsumoto M;Yamaguchi I;Sasano M;Hori M;Ikezaki K;Shimizu S;Nishiyama Y;Sato N;Tsuchiya H;Suzuki M;Minagawa H
• 通讯作者: Minagawa H

劇症型溶血性レンサ球菌感染症分離株でみられたクリンダマイシン耐性株の増加

暴发性溶血性链球菌感染的克林霉素耐药菌株增加

• 发表时间: 2011
• 作者: 池辺忠義;小黒祐子;嶋智子;奥野ルミ;大屋日登美;勝川千尋;富永潔;緒方喜久代;大西真;渡邉治雄
• 通讯作者: 渡邉治雄

Molecular Epidemiology of group A Streptococcus isolated from patients with severe invasive infections in Japan during 2004-2010

2004-2010年日本严重侵袭性感染患者分离A族链球菌的分子流行病学

• 发表时间: 2011
• 作者: Ikebe T;Katsukawa C;Ohya H;Suzuki R;Oguro Y;Tominaga K;Shima T;Isobe J;Ogata K;Okuno R;Fujimoto T;Tada Y;Okabe N;Ohnishi M;Watanabe H;The Working Group forβ-hemolytic Streptococci in Japan
• 通讯作者: The Working Group forβ-hemolytic Streptococci in Japan

劇症型溶血性レンサ球菌感染症における遺伝子発現調節因子の変異と好中球傷害

暴发性溶血性链球菌感染中基因表达调节因子的突变和中性粒细胞损伤

• 发表时间: 2010
• 作者: 阿戸学;松村隆之;池辺忠義;渡邉治雄;小林和夫
• 通讯作者: 小林和夫

Incompetence of neutrophils to invasive group A streptococcus is attributed to induction of plural virulence factors by dysfunction of a regulator.

中性粒细胞对侵入性A链球菌的无能归因于调节剂功能障碍诱导复数毒力因子。

• DOI: 10.1371/journal.pone.0003455
• 发表时间: 2008
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Ato, Manabu;Ikebe, Tadayoshi;Kawabata, Hiroki;Takemori, Toshitada;Watanabe, Haruo
• 通讯作者: Watanabe, Haruo