Visualization of influenza virus progeny RNP complex and analysis of its nucleus-to-plasma membrane transport mechanisms.

流感病毒基因组在细胞核内以核糖核蛋白复合体（vRNP）的形式复制，并被转运到细胞表面，在那里发生子代病毒粒子的出芽。本研究表明，子代vRNP的转运依赖于宿主细胞微管转运系统。后代vRNP的活细胞成像表明，vRNP信号沿微管快速移动（平均速度为0.7 ~ 1.5μm/s），但在正负方向上呈间歇性移动。

Influenza virus genome is replicated in the nucleus as ribonucleoprotein complex (vRNP) and is transported to the cell surface where the budding of progeny virion occurs. This research revealed that the trafficking of progeny vRNP depends on the host cellular microtubule transport system. Live cell imaging of progeny vRNP demonstrated that the vRNP signals moved along microtubules rapidly (the average velocity of 0.7-1.5μm/s) but intermittently in both plus and minus directions.