Development of diagnosis for asphyxia using 2-dimensional electrophoresis

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We explored a specific protein which responded to hypoxia on an animal model of asphyxia with 2-Dimentioanl electrophoresis. Mice were anaesthetized after which hypoxia was induced by neck ligation for 30 min, then were killed by decapitation, and the brains were dissected either immediately (H-0) or 60 min (H-60) after death. Control mice without ligation of the neck were killed by decapitation under anaesthesia immediately (C-0) or 60 min (C-60) after death.In forebrain, about 100 spots were observed. 2 spots were increased in H-0 and H-60. In addition, other 2 spots were increased. In cerebellum, about 100 spots were observed. 2 spots were increased in H-0 and H-60. In addition, other 2 spots were increased.These increased spots might be asphyxia response proteins.