Development of diagnosis for asphyxia using 2-dimensional electrophoresis

二维电泳窒息诊断技术的发展

• 批准号: 20790461
• 负责人: IKEMATSU Kazuya
• 金额: $ 2.75万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790461/
• 关键词: 法医病理学; 蛍光2次元電気泳動法; マウス小脳; 頚部圧迫; 窒息

We explored a specific protein which responded to hypoxia on an animal model of asphyxia with 2-Dimentioanl electrophoresis. Mice were anaesthetized after which hypoxia was induced by neck ligation for 30 min, then were killed by decapitation, and the brains were dissected either immediately (H-0) or 60 min (H-60) after death. Control mice without ligation of the neck were killed by decapitation under anaesthesia immediately (C-0) or 60 min (C-60) after death.In forebrain, about 100 spots were observed. 2 spots were increased in H-0 and H-60. In addition, other 2 spots were increased. In cerebellum, about 100 spots were observed. 2 spots were increased in H-0 and H-60. In addition, other 2 spots were increased.These increased spots might be asphyxia response proteins.

我们通过二维电泳探索了一种对窒息动物模型缺氧做出反应的特定蛋白质。将小鼠麻醉，然后通过颈部结扎诱导缺氧30分钟，然后通过断头处死，并在死亡后立即（H-0）或60分钟（H-60）解剖大脑。未结扎颈部的对照小鼠在死亡后立即(C-0)或60分钟(C-60)麻醉下断头处死。在前脑中观察到约100个斑点。 H-0和H-60中增加了2个点。此外，还增加了另外2个名额。在小脑中，观察到约 100 个斑点。 H-0和H-60中增加了2个点。此外，另外2个斑点也有所增加。这些增加的斑点可能是窒息反应蛋白。