Development of the method to investigate the mechanism of hypertensive remodeling using laser microdissection

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We sought to establish the method to selectively collect the myocardium, vessels, and perivascular infiltrating cells from the heart sections and to compare gene expression profile of each tissue component between normotensive and hypertensive hearts using laser microdissection method. Real-time RT-PCR analysis showed that BNP, a marker of the cardiomyocytes, was expressed in the myocardial samples, but not in the vascular samples, whereas alpha-SMA, a marker of smooth muscle cells, was detected only in the vascular samples. Moreover, BNP was more upregulated in stroke-prone spontaneously hypertensive rats than in WKY rats.