The development of immune regulation by genetically modified ES-DC

近年来的研究表明，树突状细胞在生理上对免疫反应有积极和消极的调节作用。对于DC的遗传修饰，我们使用先前建立的方法从小鼠胚胎干细胞体外产生DC （ES-DC）。我们尝试1)将表达胰岛源性抗原的ES-DC与免疫抑制分子一起转移到NOD小鼠中预防糖尿病，2)通过表达免疫抑制分子的ES-DC转移到异体胰岛移植后延长存活时间。这些策略也可用于治疗自身免疫性疾病和诱导移植耐受。

Recent studies showed that DCs physiologically play significant roles in regulating immune responses both positively and negatively. For genetic modification of DC, we used a previously established method to generate DC from mouse ES cells in vitro (ES-DC). We tried 1) the prevention of diabetes by transfer of ES-DC expressing islet-derived antigen along with immunosuppressive molecules in NOD mice, and 2) the prolongation of islet allograft survival by transfer of ES-DC expressing immunosuppressive molecules. These strategies may also be useful for the treatment of subjects with autoimmune diseases and for the induction of transplantation tolerance.