Prevention of bacterial translocation in small bowel transplantation and study for immune syetem of small intestine.

小肠移植中细菌移位的预防及小肠免疫系统的研究。

• 批准号: 20790959
• 负责人: HIGASHIJIMA Jun
• 金额: $ 2.75万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790959/
• 关键词: 腸虚血再灌流傷害; バクテリアルトランスロケーション小腸粘膜損傷; 炎症性サイトカイン; egr-1; 小腸虚血再灌流傷害; バクテリアルトランスロケーション; 小腸粘膜傷害; 炎症性サイトカイン、egr-1; 肝虚血再灌流傷害; HSP70

[Background] The purpose of this study was to clarify the effect of GGA to prevent the ischemia-reperfusion injury in the small intestine. [Methods] 1, Male Wistar rats were divided into two groups. Control group(n=4), was administered a vehicle 4 hour before ischemic condition. GGA group(n=4) was administered GGA (100mg/kg) 4 hour before ischemic condition. Ischemia- reperfusion injury in the small intestine was induced by 40 minute clamping of the root of superior mesenteric artery. After 60 minute reperfusion, cytokines in the blood were measured by RT-PCR. And the extent of damage of the mucosa was evaluated by immunohistochemical stain of HSP70.[Results] IL-1 was significantly lower in GGA group than control group (GGA vs cont : 1.78±0.90 vs 0.92±0.21, p<0.05).IL-6 was significantly lower in GGA group than control group (GGA vs cont : 78.5±40.0 vs 8.6±5.1, p<0.05). INF-_Y, Caspase 3 and TNT-α showed no significant changes. Egr- 1 was significantly lower in GGA group than control group(GGA vs cont 2.02±1.20 vs 0.44±0.28, p<0.05). HSP70 was overexpressed in the GGA group. [ Conclusion] HSP induced by GGA suppressed expressions of Egr-1 and inflammatory cytokines,produced by ischemia-reperfusion injury of small intestine.

【背景】本研究旨在阐明GGA对小肠缺血再灌注损伤的预防作用。【方法】1、雄性Wistar大鼠分为两组。对照组（n=4），缺血前4小时给药。GGA组（n=4）在缺血前4小时给予GGA （100mg/kg）。采用夹持肠系膜上动脉根40min的方法，诱导小肠缺血再灌注损伤。再灌注60分钟后，采用RT-PCR检测血液中细胞因子的含量。HSP70免疫组化染色评价大鼠粘膜损伤程度。[结果]GGA组IL-1水平明显低于对照组（GGA vs对照组：1.78±0.90 vs 0.92±0.21,p<0.05）。GGA组IL-6水平显著低于对照组（GGA vs对照组：78.5±40.0 vs 8.6±5.1,p<0.05）。INF-_Y、Caspase 3和TNT-α无显著变化。GGA组Egr- 1显著低于对照组（GGA vs对照组2.02±1.20 vs 0.44±0.28,p<0.05）。GGA组HSP70过表达。[结论]GGA诱导热休克蛋白抑制小肠缺血再灌注损伤产生的Egr-1和炎性细胞因子的表达。

项目成果

ラット小腸虚血再灌流傷害に対するGeranylgeranylacetone(GCA)誘導heat shock protein(HSP)の軽減効果の検討

香叶基香叶丙酮(GCA)诱导的热休克蛋白(HSP)对大鼠小肠缺血再灌注损伤的缓解作用研究

• 发表时间: 2008
• 作者: 小松正入;岩田貴;近清素也;吉川幸造;東島潤;西岡将規;栗田信浩;島田光生
• 通讯作者: 島田光生

Sonic hedgehog relates to colorectal carcinogenesis

• DOI: 10.1007/s00535-009-0110-2
• 发表时间: 2009-08
• 期刊: Journal of Gastroenterology
• 影响因子: 6.3
• 作者: K. Yoshikawa;M. Shimada;Hidenori Miyamoto;Jun Higashijima;T. Miyatani;M. Nishioka;N. Kurita;T. Iwata;H. Uehara

Graft size, donor age, and patient status are the indicators of early graft function after living donor liver transplantation

• DOI: 10.1002/lt.21462
• 发表时间: 2008-07-01
• 期刊: LIVER TRANSPLANTATION
• 影响因子: 4.6
• 作者: Yoshizumi, Tomoharu;Taketomi, Akinobu;Maehara, Yoshihiko
• 通讯作者: Maehara, Yoshihiko

Beneficial effects of Kampo medicine Inchin-ko-td on liver function and regeneration after hepatectomy in rats

汉方药 Inchin-ko-td 对肝切除术后大鼠肝功能和再生的有益作用

• 发表时间: 2008
• 期刊: Hepatol Res. 38
• 作者: Kurita N;Shimada M;Chikakiyo M;Higashijima J;Yoshikawa K;Miyamoto H;Nishioka M Iwata T.;Yoshizumi T;Ogasawara T
• 通讯作者: Ogasawara T

Effect of splenectomy on antitumor immune system in mice.

• 发表时间: 2009
• 期刊: Anticancer research
• 影响因子: 2
• 作者: Jun Higashijima;M. Shimada;Motoya Chikakiyo;T. Miyatani;K. Yoshikawa;M. Nishioka;T. Iwata;N. Kurita