Effects of general anesthetics in hippocampus of SAM mice and establishment of the safety for Anesthesia in senile dementia

全身麻醉药对SAM小鼠海马的影响及老年痴呆麻醉安全性的建立

• 批准号: 20791067
• 负责人: RIKA Sasaki
• 金额: $ 2.91万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791067/
• 关键词: 認知症; 海馬; 全身麻酔薬; シナプス伝達; シナプス伝逹

In order to investigate whether dementia modifies the anesthetic actions in the central nervous systems, we have studied effects of general anesthetics on the hippocampal synaptic transmission using the dementia model mice. Field population spikes of hippocampal CA1 pyramidal neurons were elicited in vitro using orthodromic stimulation of Schaffer collateral commissural fibers(test pulse). The recurrent inhibition was enhanced with the second stimulating electrode placed in alveus hippocampi(prepulse) to activate recurrent inhibition of CA1. The prepulses were applied as train stimuli to activate release and then depleteγ-amino-butyric acid(GABA) at presynaptic terminals of inhibitory interneurons. Sevoflurane and thiopental had greater actions on inhibitory synaptic transmission in dementia model mice than in control mice. The pre-pulse train protocol revealed that the anesthetic-induced GABA discharge was more enhanced in dementia mice than in control mice. Dementia enhances the actions of general anesthetics due to the increase in GABA release from presynaptic terminals.

为了探讨痴呆是否改变了中枢神经系统的麻醉作用，我们用痴呆模型小鼠研究了全身麻醉药对海马突触传递的影响。场群体尖峰的海马CA 1锥体神经元引起在体外使用的Schaffer侧支连合纤维的顺向刺激（测试脉冲）。将第二个刺激电极置于肺泡（前脉冲），激活CA 1的重复抑制，可增强重复抑制。前脉冲作为串刺激，激活抑制性中间神经元突触前终末的γ-氨基丁酸（GABA）释放，然后耗尽。七氟烷和硫喷妥钠对痴呆模型小鼠突触传递的抑制作用比对照小鼠更大。前脉冲序列实验表明，痴呆小鼠的GABA放电比对照小鼠的GABA放电更强。痴呆症由于突触前末梢释放GABA的增加而增强全身麻醉药的作用。

项目成果

Dementia enhances inhibitory actions of general anesthetics in hippocampal synaptic transmission

痴呆增强全身麻醉药对海马突触传递的抑制作用

• 发表时间: 2011
• 期刊: ISRN Anesthesiology
• 作者: Yamada M;Hirota K;Sasaki R;Yamazaki M
• 通讯作者: Yamazaki M

The Pentax-AWS rigid video laryngoscope : clinical assessment of performance in 320cases

Pentax-AWS硬式视频喉镜：320例临床性能评估

• 发表时间: 2008
• 期刊: Anaesthesia 63
• 作者: A Suzuki;Y Toyama;N Katsumi;T kunisawa;R Sasaki;K Hirota;JJ Henderson;H Iwasaki.;A Suzuki
• 通讯作者: A Suzuki

Senile dementia enhances the actions of general anesthetics on hippocampal synaptic transmission.

老年痴呆增强全身麻醉药对海马突触传递的作用。

• 发表时间: 2010
• 作者: Rika Sasaki;Koki Hirota;Yasuko Iwase;Mitsuaki Yamazaki.
• 通讯作者: Mitsuaki Yamazaki.

Senile dementia enhances the actions of general anesthetics on hippocampal synaptic transmission

老年痴呆增强全身麻醉药对海马突触传递的作用

• 发表时间: 2010
• 作者: Koki Hirota;Rika Sasaki;Yasuko Iwase;Mitsuaki Yamazaki.;Rika Sasaki
• 通讯作者: Rika Sasaki

ICUブック第3版(第48章)

ICU 书第三版（第 48 章）

• 发表时间: 2008
• 作者: 佐々木利佳;広田弘毅
• 通讯作者: 広田弘毅