Survival of salivary pleomorphic adenoma cells in hypoxic condition

缺氧条件下唾液多形性腺瘤细胞的存活

• 批准号: 20791331
• 负责人: MARUYAMA Satoshi
• 金额: $ 2.75万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791331/
• 关键词: 口腔病理学; 唾液腺多形性腺腫; 低酸素; HIF-1α; VEGF; pVHL; SM-AP; ACC

Salivary pleomorphic adenoma is histopathologically characaterized by its colorful stroma including myxoid one which is poorly vascularized. Pleomorphic adenoma (PA) cells embedded in such poorly-vasculaized stromata are supposed to have their own device to survive in hypoxic conditions. To understand the hypoxia-dependent manner of cellular proliferation, we determined both protein and gene expression levels of hypoxia-inducible factor 1α (HIF-1α), vascular endothelial growth factor (VEGF), and von Hippel-Lindau (vHL) protein which degrades HIF-1α in SM-AP cells originated from a pleomorphic adenoma. They showed more enhanced gene expression levels for VEGF but not for those for HIF-1α under hypoxic conditions. In contrast, HIF-1α and VEGF protein levels were kept higher in hypoxic conditions than in aerobic ones. SM-AP cells had lower expression levels for the vHL gene. On the one hand, their VEGF protein levels were kept lower in hypoxic conditions than in aerobic ones. The results indicate that PA cells are able to proliferate in the hypoxic condition because of the accumulation of HIF-1α probably due to repressed levels of vHL.

涎腺多形性腺瘤的组织病理学特征是其间质色彩鲜艳，其中黏液样间质血运较差。多形性腺瘤(PA)细胞嵌在这种血管稀少的间质中，被认为有自己的装置在低氧条件下生存。为了了解细胞增殖的低氧依赖方式，我们检测了多形性腺瘤来源的SM-AP细胞中低氧诱导因子1α、血管内皮生长因子和降解低氧诱导因子1α的von Hippel-Lindau蛋白的蛋白和基因表达水平。在低氧条件下，血管内皮生长因子的基因表达水平更高，而缺氧诱导因子-1α的基因表达水平没有明显提高。相反，缺氧条件下的缺氧诱导因子-1α和血管内皮生长因子蛋白水平高于有氧条件下的水平。SM-AP细胞VHL基因表达水平较低。一方面，低氧条件下血管内皮细胞生长因子蛋白水平低于有氧状态。结果表明，PA细胞能够在低氧条件下增殖，这可能是由于HIF-1α的积聚，可能是由于抑制了VHL的水平。

项目成果

歯学部の学生教育における細胞診実習の取り組み

牙科学院学生教育中细胞诊断培训的举措

• 发表时间: 2009
• 作者: 丸山 智;程 〓;山崎 学;小林 孝憲;朔 敬;丸山智;丸山智;丸山智
• 通讯作者: 丸山智

Keratinocyte growth factor and its associated molecules at the site of capsular invasion and vascular involvement in salivary pleomorphic adenomas

唾液腺多形性腺瘤囊膜侵犯和血管受累部位的角质形成细胞生长因子及其相关分子

• 发表时间: 2009
• 期刊: J Oral Pathol Med 38(4)
• 作者: Maruyama S;Cheng J;Yamazaki M;Liu A;Saku T
• 通讯作者: Saku T

腺様嚢胞癌におけるKGF発現と転移に関する検討

KGF在腺样囊性癌中表达及转移的研究

• 发表时间: 2009
• 作者: 丸山 智;程 〓;山崎 学;小林 孝憲;朔 敬;丸山智
• 通讯作者: 丸山智

Perlecan-rich epithelial linings as a background of proliferative potentials of keratocystic odontogenic tumor

富含基底膜的上皮衬里作为角化囊性牙源性肿瘤增殖潜力的背景

• 发表时间: 2008
• 期刊: Journal of Oral Pathology & Medicine 37
• 作者: Tsuneki M;Cheng J;Maruyama S;Ida-Yonemochi H;Nakajima M;Saku T
• 通讯作者: Saku T

唾液腺多形性腺腫の低酸素環境における増殖機構:SM-AP細胞による検討

低氧环境下唾液腺多形性腺瘤的生长机制：SM-AP细胞研究

• 发表时间: 2010
• 作者: 丸山 智;程 〓;山崎 学;朔 敬
• 通讯作者: 朔 敬