Analysis of bacterial biofilm in rat's root canal in vivo

首先，我用粪肠球菌和牙龈卟啉单胞菌在体外研究了一些形成细菌生物膜的生长条件。然后尝试在体内生长条件下在大鼠根管中制备细菌生物膜。结果，我没有在所有的样品中发现任何细菌。我认为原因是不确定的髓切除术。每个根管中留下的许多免疫电荷细胞被杀死了所有的细菌。我认为在体内大鼠根管内生物膜的形成需要持续的细菌菌群感染。

First of all, I examined some growth conditions to form bacterial biofilm in vitro, using Enterococcus faecalis and Porphyromonas gingivalis. Then I tried to make bacterial biofilm in rat's root canal under the growth condition in vivo. As the result, I could not find any bacteria in all samples. I think that the cause was uncertain pulpectomy. Many immunity charge cells left in every root canal were killed all of bacteria. I suppose that biofilm formation in rat's root canal in vivo need continuous infection from bacterial flora.