Expression of Apoptosis inhibitor gene, Survivin, in Human Oral Squamous Cell Carcinoma

凋亡抑制基因Survivin在人口腔鳞状细胞癌中的表达

• 批准号: 20791550
• 负责人: IKEDA Chihaya
• 金额: $ 2.41万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791550/
• 关键词: 歯学; 癌; 遺伝子; アポトーシス; ヒト口腔扁平上皮癌; survivin; ヒトロ腔扁平上皮癌

We received the approval of the Tokyo Dental College Ethical Review Board and we obtained its consent from 38 patients with oral squamous cell carcinoma and obtained a tumor tissue, a normal tissue. We completed extraction of the DNA in the all Also, it extracted RNA in ten people. We randomly chose three people among the RNA, and all genome cover analyzed the gene expression of the oral cavity squamous cell carcinoma patients using microarray. We randomly chose three people among the RNA, and all genome cover analyzed the gene expression of the oral cavity squamous cell carcinoma patients using Microarray. As a result, three people did not show the apoptosis association gene which they were common, and showed overexpression, a manifestation decrease. We used five oral cavity squamous cell carcinoma origin cell line in a DNA copy number of Survivin with the tumor tissue of 20 patients with oral squamous cell carcinoma, a normal tissue and analyzed gene expression of Survivin by real-time PCR. As a result, five of 20 people

我们得到了东京牙科大学伦理审查委员会的批准，我们从38名口腔鳞状细胞癌患者那里获得了同意，并获得了肿瘤组织，正常组织。我们总共完成了DNA的提取，它还提取了10个人的RNA。我们从RNA中随机抽取3人，利用基因芯片对口腔鳞癌患者的基因表达进行全基因组覆盖分析。我们从RNA中随机抽取3人，全基因组覆盖利用基因芯片分析口腔鳞癌患者的基因表达。结果，有3人没有表现出他们常见的凋亡相关基因，而是表现为过度表达，一种表现减少。采用5株口腔鳞癌来源细胞系中的Survivin DNA拷贝数与20例口腔鳞癌患者的肿瘤组织、正常组织进行实时定量聚合酶链式反应，分析Survivin基因的表达情况。结果，20个人中有5个