The specific molecular mechanism of periodontal ligament fibroblast in periodontal ligament during tooth eruption

牙齿萌出过程中牙周膜成纤维细胞的具体分子机制

• 批准号: 20791570
• 负责人: IIZAWA Futabako
• 金额: $ 1.58万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791570/
• 关键词: シグナル伝達; 細胞・組織; 再生医学; 歯学

The aim of this study is to investigate a part of molecular regulatory mechanism of formation and maintenance of the periodontal ligament. I used the tissue sample of periodontal ligament during eruption and the periodontal ligament of the erupted tooth to investigate the genes which has a remarkable difference of the expression level, cyclopedically. I investigated the functions and the expression patterns of the genes likely to regulate formation and regulation of periodontal ligament in vivo and in vitro.Fibromodulin and some other genes presented different mRNA expression level between the periodontal tissue of erupting murine molar and the periodontal tissue of the erupted murine molar. However, it was thought that we required the device of the further investigation method and the investigation about other genes because it was difficult to get reproducibility of the investigation in each cell line.

本研究旨在探讨牙周膜形成与维持的部分分子调控机制。我利用出牙时牙周韧带和出牙时牙周韧带的组织样本，研究了表达水平有显著差异的基因。我在体内和体外研究了可能调控牙周膜形成和调控的基因的功能和表达模式。纤维调节蛋白等基因在萌牙牙周组织与萌牙牙周组织之间mRNA表达水平存在差异。然而，由于很难在每个细胞系中获得调查的可重复性，因此认为我们需要进一步调查方法和其他基因调查的装置。