The specific molecular mechanism of periodontal ligament fibroblast in periodontal ligament during tooth eruption

本研究旨在探讨牙周膜形成与维持的部分分子调控机制。我利用出牙时牙周韧带和出牙时牙周韧带的组织样本，研究了表达水平有显著差异的基因。我在体内和体外研究了可能调控牙周膜形成和调控的基因的功能和表达模式。纤维调节蛋白等基因在萌牙牙周组织与萌牙牙周组织之间mRNA表达水平存在差异。然而，由于很难在每个细胞系中获得调查的可重复性，因此认为我们需要进一步调查方法和其他基因调查的装置。

The aim of this study is to investigate a part of molecular regulatory mechanism of formation and maintenance of the periodontal ligament. I used the tissue sample of periodontal ligament during eruption and the periodontal ligament of the erupted tooth to investigate the genes which has a remarkable difference of the expression level, cyclopedically. I investigated the functions and the expression patterns of the genes likely to regulate formation and regulation of periodontal ligament in vivo and in vitro.Fibromodulin and some other genes presented different mRNA expression level between the periodontal tissue of erupting murine molar and the periodontal tissue of the erupted murine molar. However, it was thought that we required the device of the further investigation method and the investigation about other genes because it was difficult to get reproducibility of the investigation in each cell line.