Research on mechanisms of neural transmission by neuron cell chip and X-ray microbeam irradiation system

点击翻译按钮获取中文摘要

A cell chip on which PC12 cells were artificially-arranged was developed by use of techniques of semiconductor device fabrication and biotechnology. Patterned layer for cellular adhesiveness was printed on a glass plate by lithography. After sterilizing the glass plate, the PC12 cells were seeded on the glass plate and cultured in an incubator. The neural differentiation of the PC12 cells was induced by nerve growth factor. Some PC12 cells on the cell chip were irradiated by X-ray microbeam. The radiobiological effects such as damage and repair in the target cell were investigated by immunofluorescence staining and patch clamp methods.