Research on mechanisms of neural transmission by neuron cell chip and X-ray microbeam irradiation system

神经元细胞芯片和X射线微束照射系统研究神经传递机制

• 批准号: 20760047
• 负责人: SATO Fuminobu
• 金额: $ 1.91万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20760047/
• 关键词: 放射線; 細胞照射; X線マイクロビーム; パッチクランプ; 放射線照射効果; 培養細胞パターニング

A cell chip on which PC12 cells were artificially-arranged was developed by use of techniques of semiconductor device fabrication and biotechnology. Patterned layer for cellular adhesiveness was printed on a glass plate by lithography. After sterilizing the glass plate, the PC12 cells were seeded on the glass plate and cultured in an incubator. The neural differentiation of the PC12 cells was induced by nerve growth factor. Some PC12 cells on the cell chip were irradiated by X-ray microbeam. The radiobiological effects such as damage and repair in the target cell were investigated by immunofluorescence staining and patch clamp methods.

利用半导体器件制造技术和生物技术，研制了一种人工排列PC12细胞的细胞芯片。通过光刻将用于细胞粘附性的图案层印刷在玻璃板上。将玻璃板灭菌后，将PC12细胞接种在玻璃板上并在培养箱中培养。神经生长因子可诱导PC 12细胞向神经分化。用X射线微束照射细胞芯片上的PC12细胞。应用免疫荧光染色和膜片钳技术研究靶细胞的放射生物学效应，如损伤和修复。

项目成果

X-ray microbeam system for irradiation of living cells

用于活细胞照射的 X 射线微束系统

• 发表时间: 2008
• 作者: T. Kuchimaru;F. Sato;Y. Aoi;Y. Kato;T. Iida
• 通讯作者: T. Iida

単一細胞照射実験のための小型マイクロX線ビーム装置

用于单细胞辐照实验的紧凑型微型 X 射线束装置

• 发表时间: 2008
• 期刊: 日本放射線安全管理学会誌 7
• 作者: 青位祐輔;口丸高弘;佐藤文信;加藤裕史;飯田敏行
• 通讯作者: 飯田敏行

Microchamber arrays for the identification of individual cells exposed to an x-ray microbeam

用于识别暴露于 X 射线微束的单个细胞的微室阵列

• 发表时间: 2008
• 期刊: Radiation and Environmental Biophysics 47
• 作者: T. Kuchimaru;F. Sato;Y. Aoi;T. Fujita;T. Ikeda;K. Shimizu;Y. Kato;T. Iida
• 通讯作者: T. Iida

Development of Cell Chip Based on Track Detector for Examination of Biological Damage by Alpha Particles

开发基于径迹探测器的细胞芯片，用于检测阿尔法粒子的生物损伤

• 发表时间: 2010
• 期刊: Journal of Nuclear Science and Technology
• 影响因子: 1.2
• 作者: Takahiro Kuchimaru;Fuminobu Sato;Souichi Tanaka;Isao Murata;Yushi Kato;Toshiyuki Iida
• 通讯作者: Toshiyuki Iida

New Radiophotoluminescence Glass Dosimeter with Specialized Radiation Sensitive Surface Layer

具有特殊辐射敏感表面层的新型放射光致发光玻璃剂量计

• 发表时间: 2010
• 期刊: Japanese Journal of Applied Physics
• 影响因子: 1.5
• 作者: Daisuke Maki;Hatsumi Kobayashi;Fuminobu Sato;Isao Murata;Yushi Kato;Takayoshi Yamamoto;Toshiyuki Iida
• 通讯作者: Toshiyuki Iida