The basic study of protective effects by carnosic acid against anthrocyclin-induced cardiotoxicity

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We evaluated the oxidant effects of doxorubicine (DOX) on murine cardio-myocytic cell line. DOX did not have cytotoxic effects on HL-1 cells (～100 ng/ml). Therefore, we have evaluated the cytotoxic effect of acetaminophen (AA), which has much more oxidant effects, instead of DXR. The cytotoxic effect of AA was reduced by the addition of carnosic acid (CA). The RNA was extracted from HL-1 cells, cultured with CA, which concentration showed the maximum anti-oxidant effect. The quantitative RT-PCR was performed, resulting in that phase II enzymes were not highly expressed significantly. These results suggest that anti-oxidant effect of CA might be independent on the expression of Phase II enzymes.