Role of alpha1 adrenoceptor activation in the hypothalamic paraventricular nucleus on the adrenomedullary outflow in rats

下丘脑室旁核α1肾上腺素受体激活对大鼠肾上腺髓质流出的作用

• 批准号: 20591370
• 负责人: OKADA Shoshiro
• 金额: $ 2.91万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591370/
• 关键词: NMDA; ノルアドレナリン神経系; アドレノセプター; トロンボキサン; 血中カテコールアミン; NMDA受容体; 視床下部室傍核; 副腎髄質系; 拘束ストレス; 生活習慣病; α1アドレノセプター; トロンボキサンA2; PLC; 副腎髄質系賦活

N-methyl-D-aspartate (NMDA) has been implicated in the regulation of several autonomic responses in the brain. The present study determined whether activation of α_1-adrenoceptors is involved in the centrally administered NMDA-induced adrenomedullary catecholamine outflow, using urethane-anesthetized rats. Perfusion of the hypothalamic paraventricular nucleus with NMDA (0.3 and 1.0mM) dose-dependently elevated both noradrenaline levels in the hypothalamic paraventricular nucleus and plasma catecholamine levels. These responses were abolished by co-administration of dizocilpine malate (MK-801, 0.1mM), a selective non-competitive antagonist of the NMDA receptor and by co-administration of (+)-S-145 (2.5mM), a selective competitive antagonist of the thromboxane A2 receptor. These results suggest that activation of central α_1-adrenoceptors is involved in the centrally administered NMDA-induced activation of adrenomedullary catecholamine outflow in rats. Furthermore, signaling cascades downstream of the α_1-adrenoceptor in the hypothalamic paraventricular nucleus may play an important role in the process.

N-甲基-D-天冬氨酸（NMDA）参与了脑中几种自主反应的调节。本实验用麻醉大鼠研究了α_1-肾上腺素能受体的激活是否参与了NMDA引起的肾上腺髓质儿茶酚胺外流。用NMDA（0.3和1.0mM）灌注下丘脑室旁核剂量依赖性地升高下丘脑室旁核中的去甲肾上腺素水平和血浆儿茶酚胺水平。这些反应被地佐环平苹果酸盐（MK-801，0.1 mM）（一种选择性非竞争性NMDA受体拮抗剂）和（+）-S-145（2.5 mM）（一种选择性竞争性血栓烷A2受体拮抗剂）共同给药所消除。这些结果提示，中枢α_1肾上腺素能受体的激活参与了NMDA引起的肾上腺髓质儿茶酚胺外流的激活。此外，下丘脑室旁核α_1受体下游的信号级联反应可能在这一过程中起重要作用。

项目成果

拘束ストレス負荷による血中カテコラミン増加におけるPPAR gammaの役割

PPAR γ 在约束应力负荷引起的血液儿茶酚胺增加中的作用

• 发表时间: 2010
• 作者: 岡田尚志郎;山口奈緒子
• 通讯作者: 山口奈緒子

Nitric oxide synthase isozymes in spinally projecting neurons are involved in CRF-induced sympathetic activation

脊髓投射神经元中的一氧化氮合酶同工酶参与 CRF 诱导的交感神经激活

• 发表时间: 2009
• 期刊: Autonomic Neuroscience : Basic and Clinical (印刷中)
• 作者: N Yamaguchi;S Okada;D Usui;K Yokotani
• 通讯作者: K Yokotani

Nitric oxide synthase isozymes in spinally projecting PVN neurons are involved in CRF-induced sympathetic activation

• DOI: 10.1016/j.autneu.2009.02.009
• 发表时间: 2009-06
• 期刊: Autonomic Neuroscience
• 作者: Naoko Yamaguchi;S. Okada;Daisuke Usui;K. Yokotani

脳室内投与したCRFは脳内α1アドレナリン作動性受容体の賦活を介して交感神経-副腎髄質系を賦活する

脑室内注射 CRF 通过激活大脑中的 α1 肾上腺素能受体来激活交感肾上腺髓质系统。

• 发表时间: 2010
• 作者: 岡田尚志郎;山口奈緒子
• 通讯作者: 山口奈緒子

Alphal-adrenoceptor activation is involved in the central N-methyl-D-aspartate-induced adrenomedullary outflow in rats.

α1-肾上腺素受体激活参与大鼠中枢 N-甲基-D-天冬氨酸诱导的肾上腺髓质流出。

• 发表时间: 2010
• 期刊: Eur.J.Pharmacol. 640
• 作者: Okada S;Yamaguchi N.
• 通讯作者: Yamaguchi N.