Restoration of self liver using transplantation of autologous cryopreserved hepatocytes.

点击翻译按钮获取中文摘要

This year we isolated human hepatocytes from resected liver specimen from patients with metastatic liver cancer. After some modification of the method of isolation, we could obtain intact human hepatocytes for transplantation. We transplanted those hepatocytes in nude mice and SCID mice.In addition, we used our original method to proliferate transplanted hepatocyte using radiation on host liver. Using those technique, after growth advantage, transplated human hepatocytes proliferate very well until replace a host liver to 95% By the time of 16 weeks. Around transplanted human hepatocytes, gap junction and glycogenesis were observed. Although auto transplantation has not been performed, concept of hepatocyte banking and retransplantation could be a good therapeutic choice for end stage liver diseases.