Screening for carcinogenesis-related proteins and diagnostic markers of pancreatic cancer by proteomic analysis

通过蛋白质组学分析筛选胰腺癌癌变相关蛋白和诊断标志物

• 批准号: 20591628
• 负责人: YOSHINO Shigefumi
• 金额: $ 3万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591628/
• 关键词: 膵癌; プロテオーム解析; 発癌関連蛋白質; 早期診断マーカー; 血清マーカー; α-1-antitripsin

Pancreatic cancer (PC) is one of the most lethal malignant tumors because of late diagnosis and the lack of response to various therapies. To identify potential biomarkers in cancerous serum from early stage PC patients, we carried out two-dimensional gel electrophoresis (2-DE) to compare the serum proteomic profiles from 45 patients with PC and 20 healthy volunteers. Two spots showed differential expression on 2-DE gels and these up-regulated protein spots were identified as α-1-antitrypsin (AAT). These protein spots were also confirmed by Western blotting. The serum isoforms of AAT might be clinically useful for PC diagnosis and monitoring.

胰腺癌(PC)是最致命的恶性肿瘤之一，由于其诊断较晚，对各种治疗方法缺乏反应。为了从早期PC患者的癌变血清中寻找潜在的生物标志物，我们采用双向凝胶电泳法(2-DE)比较了45例PC患者和20名健康志愿者的血清蛋白质组特征。2-DE凝胶上有两个差异表达的蛋白质斑点，这些上调的蛋白质斑点被鉴定为α-1-抗胰蛋白酶。Western blotting也证实了这些蛋白质斑点的存在。血清AAT亚型对PC的诊断和监测有一定的临床应用价值。

项目成果

Screening for serological biomarkers of pancreatic cancer by two-dimensional electrophoresis and liquid chromatography-tandem mass spectrometry

二维电泳和液相色谱-串联质谱法筛选胰腺癌血清学生物标志物

• 发表时间: 2011
• 期刊: Oncology Reports
• 影响因子: 4.2
• 作者: Wang Y;Kuramitsu Y;Yoshino S;Takashima M;Zhang X;Ueno T;Suzuki N;Oka M;Nakamura K;Yufeng Wang
• 通讯作者: Yufeng Wang

Screening for serological biomarkers of pancreatic cancer by two-dimensional electrophoresis and liquid chromatography-tandem mass spectrometry.

二维电泳和液相色谱-串联质谱法筛选胰腺癌血清学生物标志物。

• 发表时间: 2011
• 期刊: Oncol Rep. 26
• 作者: Wang Y;Kuramitsu Y;Yoshino S;Takashima M;Zhang X;Ueno T;Suzuki N;Oka M;Nakamura K
• 通讯作者: Nakamura K

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