Screening for carcinogenesis-related proteins and diagnostic markers of pancreatic cancer by proteomic analysis

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Pancreatic cancer (PC) is one of the most lethal malignant tumors because of late diagnosis and the lack of response to various therapies. To identify potential biomarkers in cancerous serum from early stage PC patients, we carried out two-dimensional gel electrophoresis (2-DE) to compare the serum proteomic profiles from 45 patients with PC and 20 healthy volunteers. Two spots showed differential expression on 2-DE gels and these up-regulated protein spots were identified as α-1-antitrypsin (AAT). These protein spots were also confirmed by Western blotting. The serum isoforms of AAT might be clinically useful for PC diagnosis and monitoring.