Myocardial protection for normothermic ischemia and reperfusion in immature heart : effect of mitochondria permeability transition pore inhibitor.

未成熟心脏常温缺血和再灌注的心肌保护：线粒体通透性转换孔抑制剂的作用。

• 批准号: 20591659
• 负责人: IMURA Hajime
• 金额: $ 2.91万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591659/
• 关键词: 未熟心筋; ミトコンドリア; 心筋保護; MPTP阻害剤; プロポフォール; 小児開心術; ランゲンドルフ; 開心術; 虚血; 再還流障害; MPTP; 人工心肺

Propofol, which is widely used as an anesthetic agent, inhibits the mitochondria permeability transition pore(MPTP) during reperfusion after ischemia and may work for myocardial protection. In the present study, we investigated whether propofol would protect the immature heart during normothermic ischemia and reperfusion. The study was carried out by using immature rabbit hearts in the Langendorff model. Propofol showed protective effect at concentrations of 1-4μg/ml, and the best protection was at 2μg/ml. The protection at 2μg/ml was equal to that of cyclosporine(another MPTP inhibitor) in a viewpoint of functional recovery of the heart. However, we also observed significant negative inotropic effect at 4μg/ml or higher concentrations and this side effect was crucial at 10μg/ml or higher.

异丙酚是一种广泛应用的麻醉药，它能抑制缺血再灌注时线粒体通透性转换孔（MPTP）的变化，可能具有心肌保护作用。在本研究中，我们探讨了异丙酚是否会保护未成熟的心脏在常温缺血和再灌注。本研究采用Langendorff模型的未成熟兔心脏进行。异丙酚在1-4μg/ml浓度范围内均表现出保护作用，以2μg/ml浓度保护作用最强。从心脏功能恢复的角度来看，2μg/ml的保护作用与环孢素（另一种MPTP抑制剂）相当。然而，我们还观察到4μg/ml或更高浓度下的显著负性肌力作用，并且该副作用在10μg/ml或更高浓度下至关重要。