Elucidation of mechanism of increase in oxidative stress related to intrinsic susceptibility in brain microvascular endothelial cells

通过比较脑微血管内皮细胞(BMEC)和主动脉内皮细胞(AEC)，我们研究了BMEC的内在易感性及其相关的氧化应激增加。在研究晚期糖基化终产物对分子通透性的影响时，BMEC的分子通透性比AEC更显著。BMEC的易感性依赖于活性氧过度产生所诱导的血管内皮生长因子的表达。在研究高血糖对BMEC和AEC产生活性氧的影响时，高血糖使BMEC和AEC产生的活性氧增加。由于NADPH氧化酶的一个亚基NOX4在相同的高血糖条件下也增加，我们推测NOX4与高血糖诱导的活性氧的过量产生有关。

We investigated intrinsic susceptibility and its associated increase of oxidative stress in brain microvascular endothelial cells (BMEC) by comparing BMEC and aortic endothelial cells (AEC). When investigating the effect of advanced glycation end products on molecular permeability, the molecular permeability was more prominent in BMEC than in AEC. The susceptibility in BMEC was dependent on VEGF expression induced by over-production of reactive oxygen species. When investigating the effect of hyperglycemia on production of reactive oxygen species in BMEC and AEC, the production was increased in hyperglycemia. Since NOX4, one of subunits of NADPH oxidases, was also increased in the same hyperglycemic condition, we speculate that NOX4 is related to hyperglycemia-induced over-production of reactive oxygen species.