Application of liposome decorated with sugar chains to prevent vascular stenosis after angioplasty

糖链修饰脂质体在预防血管成形术后血管狭窄中的应用

• 批准号: 20591700
• 负责人: TSURUSHIMA Hideo
• 金额: $ 2.91万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591700/
• 关键词: 脳血管内外科学; ナノ粒子; DDS; active targeting; リポソーム; 虚血性疾患; 血管形成術; 再狭窄予防; 糖鎖; シポソーム; 糖鎖-レクチン蛋白質親和性; drug delivery system; active targeting DDS; ドキソルビシン; 血管狭窄; 血管形成術後再狭窄; 血管狭窄予防

Restenosis remains a serious complication that can occur after angioplasty. This study investigated the efficiency of an active targeting chemotherapy using liposomes,including doxorubicin, which surface was decorated with sialyl Lewis X (SLX), (Dox-Lipo-SLX), to prevent stenosis after angioplasty. Its delivery was controlled via the affinity between SLX and E-selectin proteins, which are expressed on injured arteries. In vitro experiments confirmed that doxorubicin accumulated owing to Dox-Lipo-SLX by adhering to E-selectin-positive cells. Rats with balloon-injured arteries were intravenously injected with Dox-Lipo-SLX. A significant doxorubicin accumulation was observed on the injured vessel walls in rats treated with Dox-Lipo-SLX. The efficiency of stenosis prevention was evaluated. The residual lumen area of the group treated with Dox-Lipo-SLX was significantly larger than all of the other groups. These results demonstrate that an active targeting drug delivery system with Dox-Lipo-SLX effectively prevents stenosis after angioplasty.

再狭窄仍然是血管成形术后可能发生的严重并发症。本研究研究了使用脂质体（包括阿霉素）进行主动靶向化疗的效率，脂质体表面用唾液酸路易斯 X (SLX)、(Dox-Lipo-SLX) 修饰，以预防血管成形术后狭窄。它的传递是通过 SLX 和 E-选择素蛋白之间的亲和力来控制的，这两种蛋白在受伤的动脉上表达。体外实验证实，多柔比星由于 Dox-Lipo-SLX 通过粘附在 E-选择素阳性细胞上而积累。动脉球囊损伤的大鼠静脉注射 Dox-Lipo-SLX。在用 Dox-Lipo-SLX 治疗的大鼠中，在受伤的血管壁上观察到显着的阿霉素积累。评估了预防狭窄的效率。用 Dox-Lipo-SLX 治疗组的残余管腔面积显着大于所有其他组。这些结果表明，采用 Dox-Lipo-SLX 的主动靶向药物递送系统可有效预防血管成形术后的狭窄。

项目成果

Highly efficient gene transfer system using a laminin-DNA-apatite composite layer

• DOI: 10.1002/jgm.1425
• 发表时间: 2010-02-01
• 期刊: JOURNAL OF GENE MEDICINE
• 影响因子: 3.5
• 作者: Oyane, Ayako;Tsurushima, Hideo;Ito, Atsuo
• 通讯作者: Ito, Atsuo

脳虚血再灌流における酸化ストレス障害と脳保護療法 pH応答性ラジカル含有ナノ粒子 (radical-containing nanoparticle : RNP) の可能性-

脑缺血再灌注期间的氧化应激损伤和脑保护治疗 pH 响应性含自由基纳米颗粒 (RNP) 的可能性

• 发表时间: 2010
• 期刊: 脳卒中 32(6)
• 作者: 丸島愛樹;鈴木謙介;長崎幸夫;吉冨徹;藤加珠子;鶴嶋英夫;平山暁;松村明
• 通讯作者: 松村明

Application of liposome incorporating doxorubicin with sialyl Lewis X to prevent stenosis after rat carotid artery injury

阿霉素与唾液酸Lewis X脂质体在预防大鼠颈动脉损伤后狭窄中的应用

• 发表时间: 2009
• 期刊: Biomaterials 30
• 作者: Tsuruta W;Tsurushima H;et al.
• 通讯作者: et al.

Application of Doxorubicin liposome with sialyl Lewis X for the prevention of stenosis after angioplasty in the rat carotid artery

唾液酸Lewis X阿霉素脂质体在预防大鼠颈动脉血管成形术后狭窄中的应用

• 发表时间: 2008
• 作者: Tsurushima H;et al.
• 通讯作者: et al.

本研究に関して医学新聞メディカルトリビューン紙より取材を受けた(2009年3月)

我接受了医学报纸《Medical Tribune》关于这项研究的采访（2009 年 3 月）