The regenerative effect of autologous growth factors on nectotic bone.

本研究的目的是确定富血小板血浆（PRP）用于骨再生的合适浓度，并评估PRP对MSCs的影响。在体外研究中，我们发现PRP对细胞增殖具有浓度依赖性。PRP浓度分别为125x10^4/μl和62.5x10^4/ μl， MSCs向成骨细胞分化。在体内研究中，使用8 × 10^6的MSCs和2 × 10^8的血小板，可以观察到足够的骨再生。PRP可作为临床应用中安全、有效的激活剂。

The aim of this study was to determine the appropriate concentration of platelet rich plasma (PRP) for a bone regeneration and evaluate the effect of PRP on MSCs. In in vitro study, we showed that PRP had a concentration-dependent effect on the cell proliferation. MSCs differentiated into osteoblasts with 125x10^4/μl and 62.5x10^4/ μl of PRP. In in vivo study, adequate bone regeneration was observed using 8x10^6 of MSCs and 2x10^8 of platelet. PRP could be candidate for safe and useful activator in clinical application.