Developing new diagnostic kit for prostate cancer through detecting methylated DNA in urine sample

我们使用寡组芯片分析经去甲基化剂处理的前列腺癌细胞系，重点筛选APC、GSTP1和MT1G的甲基化DNA。用前列腺组织和尿液样本提取DNA，并进行甲基化特异性PCR。MT1G的甲基化状态与肿瘤分期显著相关。这些标记物可能具有区分前列腺癌和良性前列腺肥大的潜在能力。

We screened methylated DNA by using oligomicroarray analyses of prostate cancer cell lines treated by a demethylating agent and focused on APC, GSTP1, and MT1G. Prostate tissues and urine samples were used for extracting their DNA, which were subjected to methylation specific PCR. The methylation status of MT1G was significantly associated with tumor stage. These markers may have potential abilities to distinguish prostate cancer from benign prostate hypertrophy.