The establishment of a treatment system using digital imaging analysis on intracellular calcium ion dynamics of golden hamster prostatic smooth muscle cells.

利用数字成像分析金黄地鼠前列腺平滑肌细胞内钙离子动力学的治疗系统的建立。

• 批准号: 20591888
• 负责人: MATSUURA Makoto
• 金额: $ 2.58万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591888/
• 关键词: 細胞内カルシウム変動; リアルタイム共焦点レーザー顕微鏡; α1受容体; 加齢; ノルアドレナリン; 細胞内カルシウム濃度変動; Nor Adrenalin(NAd); ATP(アデノシン三リン酸); P_2X受容体; NorAdrenalin(NAd); P2X受容体

We examined the intracellular calcium ion dynamics ([Ca2+]i) in the cell in an age-related alpha receptor and the purinoceptor with real time confocal laser microscope using golden hamster to clarify the correlation of the intercellular signaling in the prostatic smooth muscle cell. The shrinkage of the prostatic smooth muscle cell became clear by calcium entry from extracellular than the analysis of the reaction pattern. After examining the local existence of alpha 1 receptor, the local existence of the clear receptor was not accepted in the smooth muscle cell by the fluorescence immunohistochemistry.

本研究采用真实的时间激光共聚焦显微镜观察了前列腺平滑肌细胞内与年龄相关的α受体和嘌呤受体的胞内钙离子动力学（[Ca ~（2+）]i），以阐明前列腺平滑肌细胞内信号转导的相关性。细胞外钙离子进入前列腺平滑肌细胞的收缩比反应模式分析更明显。荧光免疫组化法检测平滑肌细胞α 1受体的局部存在后，未发现平滑肌细胞α 1受体的局部存在。

项目成果

Comparison of effects with respect to diuretics on intracellular calcium dynamics in rat testicular arteriole smooth muscle cells.

比较利尿剂对大鼠睾丸小动脉平滑肌细胞内钙动态的影响。

• 发表时间: 2008
• 作者: Tamagawa Y;Matsuura M;Saino T;Satoh Y
• 通讯作者: Satoh Y

Ca^<2+> imaging revealed regional differences of 5-HT receptors in cerebral and testicular arterioles of rats : dependence on arteries size and organ specificity.

Ca ^ 2 成像揭示了大鼠脑和睾丸小动脉中5-HT受体的区域差异：依赖于动脉大小和器官特异性。

• 发表时间: 2008
• 作者: Masu K;Saino T;Kuroda T;Matsuura M;Russa AD;Ishikita N;Satoh Y
• 通讯作者: Satoh Y

The effect of taurine, a novel biochemical modulator, on the antitumor activity of Doxorubicin.

牛磺酸（一种新型生化调节剂）对阿霉素抗肿瘤活性的影响。

• 发表时间: 2009
• 期刊: Biol Pharm Bull. 32(9)
• 作者: Sadzuka Y;Matsuura M;Sonobe T
• 通讯作者: Sonobe T

Age-associated change in the reactivity of Noradrenalin-induced intracellular calcium dynamics in prostatic smooth muscles

前列腺平滑肌去甲肾上腺素诱导的细胞内钙动力学反应性与年龄相关的变化

• 发表时间: 2009
• 作者: Matsuura M;Kuroda T;Tamagawa Y;Saino T;Satoh Y;Sadzuka Y
• 通讯作者: Sadzuka Y

ATP-induced intracellular calcium dynamics in prostatic smooth muscles of golden hamsters.

ATP 诱导的金仓鼠前列腺平滑肌细胞内钙动态。

• 发表时间: 2008
• 作者: Matsuura M;Kuroda T;Saino T;Satoh Y
• 通讯作者: Satoh Y