Analysis of regulatory mechanism of gene expression of sex steroid hormone receptors using somatic cell nuclear transfer technique

本研究的目的是利用体细胞核移植（SCNT）技术研究性类固醇激素受体，特别是雌激素受体α基因表达的调控机制。然而，在本项目的第一年和第二年的研究表明，由于技术的不成熟，SCNT技术很难应用于研究。因此，我在这个项目中专注于技术的改进。结果表明，冻融卵浆可首次用于SCNT。

The aim of this study is to investigate the regulatory mechanism of gene expression of sex steroid hormone receptors, especially estrogen receptor alpha, using the somatic cell nuclear transfer (SCNT) technique. However, the studies in the first and second years of this project revealed that the SCNT technique is difficult to be applied on the study because of immaturity of the technique. Thus, I focused on the improvement of the technique in this project. As the result, I have revealed that the frozen-thawed ooplasm can be used for the SCNT for the first time.