Establishment of serum Heparanase concentration measurement method and its application to gynecologic cancer treatment

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To establish a measurement method of serum heparanase titer, we constructed ELISA system using antiHPR1 antibody, synthesized peptide for heparanase, and biotin-labeled peptide. The ELISA for heparanase measurement worked well in the reaction of the antibody and the synthesized peptide between concentrations of 10 to 1,000 nmol/l of HPR1 peptide. The ELISA also worked well in the measurement of serum heparanase between concentrations of 1 to 100nmol/l of HPR1 peptide. We tried to measure the 22 serum samples from patients with cervical cancer, endometrial cancer and ovarian cancer, and were able to get appropriate titers of heparanase concentration in each sample. These data indicate that our ELISA system for the measurement of serum heparanase titer is applicable for a clinical setting.