Investigation of pathology of age-related hearing loss and noise-induced hearing loss and application for treatment using gene modification animals

C57 BL/6小鼠是增龄性感音神经性耳聋的动物模型。我们表明，随着年龄的增长，核酸的氧化应激标记物的表达增强，氧化应激被认为是增加。蛋白质组学分析显示，减少的相关蛋白质和抗氧化酶。利用内耳器官培养，我们建立了一种方法来评估信号转导后的生长因子管理的免疫组织化学。

The C57BL/6 mouse is used as animal models of the sensorineural deafness by the aging widely. We show the expression of oxidative stress marker reinforcement of the nucleic acid with aging, and oxidative stress is considered to increase. Proteomics analysis revealed the decrease of mitochondria-related proteins and antioxidant enzymes. Using organ culture of the inner ear, we established a method to evaluate signal transduction after the growth factors administration by immunohistochemistry.